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Addgene

pcDNA5/FRT/TO_b4g-STf
(Plasmid #182005)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182005 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    b4g-STf
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    770
  • Mutation
    Fast mutation on SNAP-tag E30R
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO_b4g-STf was a gift from Kai Johnsson (Addgene plasmid # 182005 ; http://n2t.net/addgene:182005 ; RRID:Addgene_182005)

  • For your References section:

    Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes. Frei MS, Koch B, Hiblot J, Johnsson K. ACS Chem Biol. 2022 Jun 17;17(6):1321-1327. doi: 10.1021/acschembio.2c00041. Epub 2022 May 18. 10.1021/acschembio.2c00041 PubMed 35584304
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