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Addgene

Friend MLV Env YFP
(Plasmid #1820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Envelope
  • Alt name
    Env
  • Alt name
    FrMLVgp3
  • Species
    MuLV
  • Insert Size (bp)
    2800
  • Entrez Gene
    env (a.k.a. FrMLVgp3)
  • Tag / Fusion Protein
    • EYFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ? (unknown if destroyed)
  • 3′ cloning site ? (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Abraham Pinter

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

YFP was cloned into the NheI site of pFr-MuLV273/274 from Abraham Pinter. The entire Env gene was then transferred into pcDNA3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Friend MLV Env YFP was a gift from Walther Mothes (Addgene plasmid # 1820 ; http://n2t.net/addgene:1820 ; RRID:Addgene_1820)

  • For your References section:

    Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W. Traffic 2003 Nov;4(11):785-801. 10.1034/j.1600-0854.2003.00135.x PubMed 14617360
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