Skip to main content
Addgene

pcDNA3.1(+)-mCherry-p115(S240A)
(Plasmid #181997)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181997 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5377
  • Total vector size (bp) 8824
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-p115(S240A)
  • Alt name
    P115-RHOGEF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3447
  • Mutation
    Serine 240 changed to alanine in p115.
  • Entrez Gene
    ARHGEF1 (a.k.a. GEF1, IMD62, LBCL2, LSC, P115-RHOGEF, SUB1.5)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-mCherry-p115(S240A) was a gift from Jin Zhang (Addgene plasmid # 181997 ; http://n2t.net/addgene:181997 ; RRID:Addgene_181997)

  • For your References section:

    Histamine-induced biphasic activation of RhoA allows for persistent RhoA signaling. Zhang JZ, Nguyen AH, Miyamoto S, Heller Brown J, McCulloch AD, Zhang J. PLoS Biol. 2020 Sep 3;18(9):e3000866. doi: 10.1371/journal.pbio.3000866. eCollection 2020 Sep. 10.1371/journal.pbio.3000866 PubMed 32881857
Related items:
Commonly requested with: