H2B-CoilE-mEm
(Plasmid
#181992)
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PurposeCoilE genetically encoded tag on H2B-mEmerald, to be used with CoilR labeled with a reporter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonemEmerald-H2B-6
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 5863
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-CoilE-mEm
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- mEmerald (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2B-CoilE-mEm was a gift from Kimberly Beatty (Addgene plasmid # 181992 ; http://n2t.net/addgene:181992 ; RRID:Addgene_181992) -
For your References section:
VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Doh JK, White JD, Zane HK, Chang YH, Lopez CS, Enns CA, Beatty KE. Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):12961-12966. doi: 10.1073/pnas.1808626115. Epub 2018 Dec 5. 10.1073/pnas.1808626115 PubMed 30518560
