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Addgene

H2B-MiniE-mEm
(Plasmid #181991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181991 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mEmerald-H2B-6
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 5863
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-MiniE-mEm
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • mEmerald (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H2B-MiniE-mEm was a gift from Kimberly Beatty (Addgene plasmid # 181991 ; http://n2t.net/addgene:181991 ; RRID:Addgene_181991)

  • For your References section:

    MiniVIPER Is a Peptide Tag for Imaging and Translocating Proteins in Cells. Doh JK, Tobin SJ, Beatty KE. Biochemistry. 2020 Aug 25;59(33):3051-3059. doi: 10.1021/acs.biochem.0c00526. Epub 2020 Aug 17. 10.1021/acs.biochem.0c00526 PubMed 32786411
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