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pET28b-CoilR-Cys
(Plasmid #181989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21-DE3*
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CoilR-Cys
  • Alt name
    CoilR
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer T7-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-CoilR-Cys was a gift from Kimberly Beatty (Addgene plasmid # 181989 ; http://n2t.net/addgene:181989 ; RRID:Addgene_181989)

  • For your References section:

    VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy. Doh JK, White JD, Zane HK, Chang YH, Lopez CS, Enns CA, Beatty KE. Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):12961-12966. doi: 10.1073/pnas.1808626115. Epub 2018 Dec 5. 10.1073/pnas.1808626115 PubMed 30518560
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