pcDNA TfR1-miniE
(Plasmid
#181987)
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PurposeMiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTfR1-MiniE
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA TfR1-miniE was a gift from Kimberly Beatty (Addgene plasmid # 181987 ; http://n2t.net/addgene:181987 ; RRID:Addgene_181987) -
For your References section:
MiniVIPER Is a Peptide Tag for Imaging and Translocating Proteins in Cells. Doh JK, Tobin SJ, Beatty KE. Biochemistry. 2020 Aug 25;59(33):3051-3059. doi: 10.1021/acs.biochem.0c00526. Epub 2020 Aug 17. 10.1021/acs.biochem.0c00526 PubMed 32786411
