Skip to main content
Addgene

pET28b-miniR-DBCO
(Plasmid #181984)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181984 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21-DE3*
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MiniR-DBCO
  • Alt name
    MiniR
  • Species
    Synthetic
  • Tag / Fusion Protein
    • DBCO-Tag, His-Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7-F
  • 3′ sequencing primer T7 REV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-miniR-DBCO was a gift from Kimberly Beatty (Addgene plasmid # 181984 ; http://n2t.net/addgene:181984 ; RRID:Addgene_181984)

  • For your References section:

    MiniVIPER Is a Peptide Tag for Imaging and Translocating Proteins in Cells. Doh JK, Tobin SJ, Beatty KE. Biochemistry. 2020 Aug 25;59(33):3051-3059. doi: 10.1021/acs.biochem.0c00526. Epub 2020 Aug 17. 10.1021/acs.biochem.0c00526 PubMed 32786411
Related items:
Commonly requested with: