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pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR
(Plasmid #181975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR v2
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FMC6.3-BBz CAR; tNGFR
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    NGFR truncation: aa 11-276
  • Entrez Gene
    NGFR (a.k.a. CD271, Gp80-LNGFR, TNFRSF16, p75(NTR), p75NTR)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR was a gift from Neville Sanjana (Addgene plasmid # 181975 ; http://n2t.net/addgene:181975 ; RRID:Addgene_181975)

  • For your References section:

    A genome-scale screen for synthetic drivers of T cell proliferation. Legut M, Gajic Z, Guarino M, Daniloski Z, Rahman JA, Xue X, Lu C, Lu L, Mimitou EP, Hao S, Davoli T, Diefenbach C, Smibert P, Sanjana NE. Nature. 2022 Mar;603(7902):728-735. doi: 10.1038/s41586-022-04494-7. Epub 2022 Mar 16. 10.1038/s41586-022-04494-7 PubMed 35296855
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