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Addgene

secCitrine
(Plasmid #181964)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181964 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAD
  • Backbone manufacturer
    DeBono 2011
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6500
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    pAD is based on pGreenII 0229. The pSOUP helper is required for transformation of plant tissue.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    signal peptide sequence from basic chitinase
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    102
  • GenBank ID
    At3g12500
  • Promoter 1.5 kb MUM4 promoter
  • Tag / Fusion Protein
    • Citrine YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer MUM4 promoter For
  • 3′ sequencing primer Citrine Rev GGACACGCTGAACTTGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    secCitrine was a gift from George Haughn (Addgene plasmid # 181964 ; http://n2t.net/addgene:181964 ; RRID:Addgene_181964)

  • For your References section:

    Asymmetric distribution of extracellular matrix proteins in seed coat epidermal cells of Arabidopsis is determined by polar secretion. Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW. Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. eCollection 2021 Nov. 10.1002/pld3.360 PubMed 34877448
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