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Addgene

cASPG1-mRFP
(Plasmid #181962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    R4pGWB553
  • Backbone manufacturer
    Tsuyoshi NAKAGAWA, Shinya NAKAMURA, Katsunori TANAKA, Makoto KAWAMUKAI, Takamasa SUZUKI, Kenzo NAKAMURA, Tetsuya KIMUR
  • Backbone size w/o insert (bp) 12000
  • Total vector size (bp) 14800
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ASPG1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1500
  • GenBank ID
    At3g18490
  • Entrez Gene
    ASPG1 (a.k.a. AT3G18490, ASPARTIC PROTEASE IN GUARD CELL 1)
  • Promoter TBA2 promoter
  • Tag / Fusion Protein
    • mRFP (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cASPG1-mRFP was a gift from George Haughn (Addgene plasmid # 181962 ; http://n2t.net/addgene:181962 ; RRID:Addgene_181962)

  • For your References section:

    Asymmetric distribution of extracellular matrix proteins in seed coat epidermal cells of Arabidopsis is determined by polar secretion. Lee YC, Dean GH, Gilchrist E, Tsai AY, Haughn GW. Plant Direct. 2021 Nov 28;5(11):e360. doi: 10.1002/pld3.360. eCollection 2021 Nov. 10.1002/pld3.360 PubMed 34877448
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