pcDNA3-EGFP-AC8(1-106)
(Plasmid #181958)

• Purpose: Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.
• Depositing Lab: Jin Zhang
• Publication: Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 10.7554/eLife.55013.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5430
• Total vector size (bp): 6549
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP-AC8(1-106)
• Species: R. norvegicus (rat)
• Insert Size (bp): 1119
• Entrez Gene: Adcy8 (a.k.a. Ac8)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag (N terminal on backbone)
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-EGFP-AC8(1-106).gb
• A portion of this plasmid was derived from a plasmid made by: Rat Adcy8 cDNA was provided by Dermot M.F. Cooper (University of Cambridge).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry