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Addgene

pKB083
(Plasmid #181952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a
  • Backbone size w/o insert (bp) 1885
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PhoQ; lacI
  • Species
    PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli
  • GenBank ID
    PhoQ - 1252748; lacI - 945007
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)
  • Promoter PhoQ-Ptac; lacI-PlacIq

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a S286L mutation in lacI. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKB083 was a gift from Jeffrey Tabor (Addgene plasmid # 181952 ; http://n2t.net/addgene:181952 ; RRID:Addgene_181952)

  • For your References section:

    Real-time detection of response regulator phosphorylation dynamics in live bacteria. Butcher RJ, Tabor JJ. Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22. 10.1073/pnas.2201204119 PubMed 35994658
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