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Addgene

pKD317.8
(Plasmid #181951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15a
  • Backbone size w/o insert (bp) 1885
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NarX; lacI
  • Species
    NarX - E. coli; lacI - E. coli
  • GenBank ID
    NarX - 945788; lacI - 945007
  • Entrez Gene
    narX (a.k.a. b1222, ECK1216, frd1, frdR, narR)
  • Entrez Gene
    lacI (a.k.a. b0345, ECK0342)
  • Promoter NarX-Ptac; lacI-PlacIq

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD317.8 was a gift from Jeffrey Tabor (Addgene plasmid # 181951 ; http://n2t.net/addgene:181951 ; RRID:Addgene_181951)

  • For your References section:

    Real-time detection of response regulator phosphorylation dynamics in live bacteria. Butcher RJ, Tabor JJ. Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22. 10.1073/pnas.2201204119 PubMed 35994658
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