pRB117.2
(Plasmid #181949)

• Purpose: aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
• Depositing Lab: Jeffrey Tabor
• Publication: Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22.

Backbone

• Vector backbone: ColE1
• Backbone size w/o insert (bp): 1706
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mNG-NarL; mCherry; tetR
• Species: Synthetic; narL - E. coli
• Mutation: See Depositor Comments Below
• GenBank ID: NarL - 945795; mNG - QIK01997; mCherry - UFQ89828; tetR - WP_000470728
• Entrez Gene: narL (a.k.a. b1221, ECK1215, frdR, narR)
• Entrez Gene: AX04_RS23485 (a.k.a. AX04_RS23485, AX04_23495)
• Promoter: mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106
• Tag / Fusion Protein:
  • mNeonGreen (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsaI (destroyed during cloning)
• 3′ cloning site: BsaI (destroyed during cloning)

Resource Information

• Supplemental Documents:
  • prb117-2.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains an E7G mutation in mNeonGreen. This mutation is in the unstructured N-terminal region prior to the classic fluorescent protein beta-barrel shape and is not known to affect plasmid function.