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Addgene

pRB121
(Plasmid #181945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181945 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Backbone size w/o insert (bp) 1706
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mNeonGreen-mNeonGreen; tetR
  • Alt name
    mNG-mNG
  • Species
    Synthetic
  • Mutation
    See Depositor Comments Below
  • GenBank ID
    mNG - QIK01997; tetR - WP_000470728
  • Entrez Gene
    AX04_RS23485 (a.k.a. AX04_RS23485, AX04_23495)
  • Promoter mNG-mNG-PLtetO-1; tetR-J23106

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains E7G and E253G mutations in the mNeonGreen-mNeonGreen insert. These mutations are in the unstructured N-terminal region prior to the classic fluorescent protein beta-barrel shape and are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB121 was a gift from Jeffrey Tabor (Addgene plasmid # 181945 ; http://n2t.net/addgene:181945 ; RRID:Addgene_181945)

  • For your References section:

    Real-time detection of response regulator phosphorylation dynamics in live bacteria. Butcher RJ, Tabor JJ. Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22. 10.1073/pnas.2201204119 PubMed 35994658
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