pRB035
(Plasmid
#181944)
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PurposeaTc-inducible expression of mNeonGreen
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 181944 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Backbone size w/o insert (bp) 1706
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen; tetR
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Alt namemNG
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SpeciesSynthetic
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Insert Size (bp)2
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GenBank IDmNG - QIK01997; tetR - WP_000470728
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Entrez GeneAX04_RS23485 (a.k.a. AX04_RS23485, AX04_23495)
- Promoter mNG-PLtetO-1; tetR-J23106
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTwist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRB035 was a gift from Jeffrey Tabor (Addgene plasmid # 181944 ; http://n2t.net/addgene:181944 ; RRID:Addgene_181944) -
For your References section:
Real-time detection of response regulator phosphorylation dynamics in live bacteria. Butcher RJ, Tabor JJ. Proc Natl Acad Sci U S A. 2022 Aug 30;119(35):e2201204119. doi: 10.1073/pnas.2201204119. Epub 2022 Aug 22. 10.1073/pnas.2201204119 PubMed 35994658