pC0037
(Plasmid
#181934)
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PurposeDual secreted luciferase reporter (EF1alpha-Gaussia luciferase, CMV-Cypridina luciferase)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameGaussia luciferase
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SpeciesGaussia princeps
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Insert Size (bp)555
- Promoter EF1alpha
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
- 5′ sequencing primer catttcaggtgtcgtgacGTACGggatcct (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCypridina luciferase
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SpeciesCypridina noctiluca
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Insert Size (bp)1659
- Promoter CMV
Cloning Information for Gene/Insert 2
- Cloning method Gibson Cloning
- 5′ sequencing primer aatacgactcactataggg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC0037 was a gift from Feng Zhang (Addgene plasmid # 181934 ; http://n2t.net/addgene:181934 ; RRID:Addgene_181934) -
For your References section:
RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703
