YFP-CD82
(Plasmid #1819)

• Depositing Lab: Walther Mothes
• Publication: Sherer et al Traffic 2003 Nov;4(11):785-801.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: BD Biosciences / Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD82
• Alt name: tetraspanin CD82
• Alt name: KAI1
• Species: H. sapiens (human)
• Insert Size (bp): 700
• Entrez Gene: CD82 (a.k.a. 4F9, C33, GR15, IA4, KAI1, R2, SAR2, ST6, TSPAN27)
• Tag / Fusion Protein:
  • YFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Peter Cresswell provided CD82
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry