pPHR-mCherry-mHP1alpha
(Plasmid #181883)

• Purpose: Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers
• Depositing Lab: Karsten Rippe
• Publication: Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25.

Backbone

• Vector backbone: pmCherry-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3958
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHR-mCherry-mHP1alpha
• Alt name: PHR domain of CRY2
• Alt name: CBX 5
• Species: M. musculus (mouse), A. thaliana (mustard weed)
• Entrez Gene: Cbx5 (a.k.a. 2610029O15Rik, HP1, Hp1a, Hp1alpha)
• Promoter: CMV
• Tag / Fusion Protein:
  • PHR-mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned mouse HP1a into PHR-mCherry vector and introduced another cloning site between mCherry and mHP1a to quickly insert droplet forming proteins (e. g. FUSN, PGL-1, RGG...)