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Addgene

pAAV-shNCLX-1
(Plasmid #181870)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181870 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-U6_CaMKIIa-mCherry
  • Vector type
    Mammalian Expression, Mouse Targeting, Adenoviral, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    solute carrier 8 family member B1
  • Alt name
    NCLX
  • Alt name
    sodium/calcium/lithium exchanger
  • gRNA/shRNA sequence
    ATGTTGGACTGTGGATCTAAA
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_133221
  • Entrez Gene
    Slc8b1 (a.k.a. AF261233, NCKX6, NCLX, Slc24a6)
  • Promoter U6
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamhI (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer TTGGGTAGTTTGCAGTTTTAAAATT
  • 3′ sequencing primer TCACTGAGGAAGCTCTCTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-shNCLX-1 was a gift from Hilmar Bading (Addgene plasmid # 181870 ; http://n2t.net/addgene:181870 ; RRID:Addgene_181870)

  • For your References section:

    Disrupted expression of mitochondrial NCLX sensitizes neuroglial networks to excitotoxic stimuli and renders synaptic activity toxic. Hagenston AM, Yan J, Bas-Orth C, Tan Y, Sekler I, Bading H. J Biol Chem. 2021 Dec 20:101508. doi: 10.1016/j.jbc.2021.101508. 10.1016/j.jbc.2021.101508 PubMed 34942149
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