Lyn-FRB-GFP11
(Plasmid #181863)

• Purpose: Plasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.
• Depositing Lab: Jin Zhang
• Publication: Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/eabe4091. doi: 10.1126/sciadv.abe4091. Print 2021 May.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5415
• Total vector size (bp): 5817
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-FRB-GFP11
• Insert Size (bp): 402
• Promoter: CMV
• Tags / Fusion Proteins:
  • N-terminal targeting sequence from Lyn kinase (N terminal on insert)
  • GFP11 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-Lyn-FRB-GFP11.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry