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Addgene

pcDNA3-FluoSTEP-AKAR (C/Y)
(Plasmid #181862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5377
  • Total vector size (bp) 7312
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FluoSTEP-AKAR (C-Y)
  • Alt name
    cpVenus(E172)-FHA1-PKAsub-CFP(1-10)
  • Insert Size (bp)
    1935
  • Promoter CMV
  • Tags / Fusion Proteins
    • cpVenus(E127) (N terminal on insert)
    • CFP(1-10) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FluoSTEP-AKAR (C/Y) was a gift from Jin Zhang (Addgene plasmid # 181862 ; http://n2t.net/addgene:181862 ; RRID:Addgene_181862)

  • For your References section:

    FluoSTEPs: Fluorescent biosensors for monitoring compartmentalized signaling within endogenous microdomains. Tenner B, Zhang JZ, Kwon Y, Pessino V, Feng S, Huang B, Mehta S, Zhang J. Sci Adv. 2021 May 21;7(21). pii: 7/21/eabe4091. doi: 10.1126/sciadv.abe4091. Print 2021 May. 10.1126/sciadv.abe4091 PubMed 34020947
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