pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry
(Plasmid #181851)

• Purpose: mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.
• Depositing Lab: Jin Zhang
• Publication: Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 10.1016/j.cell.2020.07.043. Epub 2020 Aug 25.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5360
• Total vector size (bp): 7304
• Vector type: Mammalian Expression
• Selectable markers: Bleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DnaJB1-PKAcat(K72H)-mCherry
• Alt name: DnaJB1(1-70)-PKAcat(16-351;K72H)-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 1944
• Mutation: Lysine 128 in the DnaJB1-PKAcat fusion changed to histidine. Corresponds to K72H in WT PKAcat.
• Entrez Gene: PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
• Entrez Gene: DNAJB1 (a.k.a. HSPF1, Hdj1, Hsp40, RSPH16B, Sis1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry