pcDNA3-RIα-ICUE4
(Plasmid
#181848)
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PurposeICUE4 cAMP sensor tethered to the PKA RIα subunit.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 181848 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5403
- Total vector size (bp) 10233
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRIα-ICUE4
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Alt nameRIα-ECFP-Epac1(149-881; Q270E)-cpVenus(L194)
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SpeciesH. sapiens (human)
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Insert Size (bp)4830
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MutationGlutamine 122 in Epac1 domain mutated to glutamate (corresponds to Q270E in full-length Epac).
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Entrez GeneRAPGEF3 (a.k.a. CAMP-GEFI, EPAC, EPAC1, HSU79275, bcm910)
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Entrez GenePRKAR1A (a.k.a. ACRDYS1, ADOHR, CAR, CNC, CNC1, PKR1, PPNAD1, PRKAR1, Prkar1alpha, TSE1)
- Promoter CMV
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- cpVenus(L194) (C terminal on insert)
- PKA RIα (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-RIα-ICUE4 was a gift from Jin Zhang (Addgene plasmid # 181848 ; http://n2t.net/addgene:181848 ; RRID:Addgene_181848) -
For your References section:
Phase Separation of a PKA Regulatory Subunit Controls cAMP Compartmentation and Oncogenic Signaling. Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke M, Rangamani P, Taylor SS, Mehta S, Zhang J. Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 10.1016/j.cell.2020.07.043. Epub 2020 Aug 25. 10.1016/j.cell.2020.07.043 PubMed 32846158