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pcDNA3-Lyn-PlcR(K30/32L)
(Plasmid #181831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 7377
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyn-PlcR (K30L,K32L)
  • Alt name
    Lyn-EYFP-PH(PLCdelta; K30L, K32L)-pseudoligand-ECFP
  • Insert Size (bp)
    2007
  • Mutation
    Lysines 30 and 32 in PH domain mutated to leucine.
  • Promoter CMV
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • EYFP (C terminal on insert)
    • N-terminal targeting sequence from Lyn kinase (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Lyn-PlcR(K30/32L) was a gift from Jin Zhang (Addgene plasmid # 181831 ; http://n2t.net/addgene:181831 ; RRID:Addgene_181831)

  • For your References section:

    Fluorescent Biosensors for Multiplexed Imaging of Phosphoinositide Dynamics. Hertel F, Li S, Chen M, Pott L, Mehta S, Zhang J. ACS Chem Biol. 2020 Jan 17;15(1):33-38. doi: 10.1021/acschembio.9b00691. Epub 2020 Jan 6. 10.1021/acschembio.9b00691 PubMed 31855412
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