pAAV-G-Creon shRNA[Control]
(Plasmid
#181824)
-
Purpose(Empty Backbone) This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181824 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAV-EF1a-DIO-EGFP
- Backbone size (bp) 5500
-
Vector typeMammalian Expression, AAV, RNAi
- Promoter EF1a/U6
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-G-Creon shRNA[Control] was a gift from Eun Mi Hwang (Addgene plasmid # 181824 ; http://n2t.net/addgene:181824 ; RRID:Addgene_181824) -
For your References section:
Improved AAV vector system for cell-type-specific RNA interference. Kim SC, Kim A, Park JY, Hwang EM. J Neurosci Methods. 2021 Dec 21:109452. doi: 10.1016/j.jneumeth.2021.109452. 10.1016/j.jneumeth.2021.109452 PubMed 34953938
