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Addgene

pCMV-MMLVgag-3xNES-ABE8e-NG
(Plasmid #181754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 181754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3-like
  • Backbone size w/o insert (bp) 4757
  • Total vector size (bp) 11365
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MMLVgag-3xNES-ABE8e-NG
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    6609
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer ACCCTGGTCATCATCCTG
  • 3′ sequencing primer CAGGAAACAGCTATGAC; CCTTCCGAGTGAGAGAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-MMLVgag-3xNES-ABE8e-NG was a gift from David Liu (Addgene plasmid # 181754 ; http://n2t.net/addgene:181754 ; RRID:Addgene_181754)

  • For your References section:

    Engineered virus-like particles for efficient in vivo delivery of therapeutic proteins. Banskota S, Raguram A, Suh S, Du SW, Davis JR, Choi EH, Wang X, Nielsen SC, Newby GA, Randolph PB, Osborn MJ, Musunuru K, Palczewski K, Liu DR. Cell. 2022 Jan 7. pii: S0092-8674(21)01484-7. doi: 10.1016/j.cell.2021.12.021. 10.1016/j.cell.2021.12.021 PubMed 35021064
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