pCMV-MMLVgag-3xNES-Cas9
(Plasmid #181752)

• Purpose: MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.
• Depositing Lab: David Liu
• Publication: Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)01484-7. doi: 10.1016/j.cell.2021.12.021.

Backbone

• Vector backbone: pCDNA3-like
• Backbone size w/o insert (bp): 4757
• Total vector size (bp): 10783
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MMLV-Gag-3xNES-Cas9 (Spy)
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 4757
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ACCCTGGTCATCATCCTG
• 3′ sequencing primer: CAGGAAACAGCTATGAC; CCTTCCGAGTGAGAGAC

Resource Information

• Supplemental Documents:
  • v4 Cas9 nuclease VLP.gb
• A portion of this plasmid was derived from a plasmid made by: the backbone was amplified from BIC-Gag-CAS9 (Plasmid #119942)
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry