pUC19-SpRYgest_targetplasmid (KAC833)
(Plasmid
#181748)
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Purpose(Empty Backbone) pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 181748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Vector typesubstrate plasmid
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer os280-CAGGGTTATTGTCTCATGAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
KAC833: EMX1 site 1 target site with NGG PAM (between NheI and HindIII sites)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUC19-SpRYgest_targetplasmid (KAC833) was a gift from Benjamin Kleinstiver (Addgene plasmid # 181748 ; http://n2t.net/addgene:181748 ; RRID:Addgene_181748) -
For your References section:
Precise DNA cleavage using CRISPR-SpRYgests. Christie KA, Guo JA, Silverstein RA, Doll RM, Mabuchi M, Stutzman HE, Lin J, Ma L, Walton RT, Pinello L, Robb GB, Kleinstiver BP. Nat Biotechnol. 2022 Oct 6. pii: 10.1038/s41587-022-01492-y. doi: 10.1038/s41587-022-01492-y. 10.1038/s41587-022-01492-y PubMed 36203014
Map uploaded by the depositor.
