pHIV-Zsgreen
(Plasmid #18121)

• Depositing Labs: Bryan Welm, Zena Werb
• Publication: Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102.

Backbone

• Vector backbone: pSICO
• Backbone size w/o insert (bp): 6900
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Zsgreen
• Alt name: Zoanthus sp. green fluorescent protein
• Species: Anthozoa
• Insert Size (bp): 700
• GenBank ID: AF168422

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MscI (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Zsgreen is from Clontech and pSICO is from Tyler Jacks.
• Articles Citing this Plasmid:
  • 32 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HIV-Zsgreen was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox- WPRE content of pSICO (Ventura et al., 2004), and adding the EF1-alpha promoter, a multiple cloning site (MCS), an internal ribosome entry site (IRES) and Zsgreen. cDNA can be cloned into the MCS (NotI, EcoRI, HpaI, XbaI, SmaI, and BamHI sites) to enable bicistronic expression with Zsgreen.