MLV Gag-CFP
(Plasmid #1812)

• Depositing Lab: Walther Mothes
• Publication: Sherer et al Traffic 2003 Nov;4(11):785-801.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gag
• Species: MuLV
• Insert Size (bp): 2800
• Entrez Gene: gag (a.k.a. FrMLVgp1)
• Tag / Fusion Protein:
  • CFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ??? (unknown if destroyed)
• 3′ cloning site: ??? (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pMDoldGag-Pol provided by Richard Mulligan

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a CFP-fusion to the amino acids PQ at the C-terminus of the Gag NC domain. This was cloned by replacing Pol with CFP in pMDoldGag-Pol.