MSCV h c-MYC IRES GFP
(Plasmid #18119)

• Depositing Lab: John Cleveland
• Publication: Cleveland lab plasmids (unpublished)

Backbone

• Vector backbone: MSCV IRES GFP
• Backbone manufacturer: Dr. Robert Hawley of George Washington University
• Backbone size w/o insert (bp): 5660
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: c-MYC
• Alt name: pMIG-cMYC
• Species: H. sapiens (human)
• Insert Size (bp): 1858
• Mutation: The CUG which is the start site for Myc I is present, however, there is a 3bp deletion between this and the ATG start site for Myc II.
• GenBank ID: NM_002467
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • MSCV-h-cMyc-IRES-GFP seq
• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: The MSCV plasmid is owned and was provided by Dr. Robert Hawley of George Washington University. The IRES-GFP DNA was developed by Dr. Arthur Nienhuis of St. Jude Children's Research Hospital (SJCRH). Dr. John L. Cleveland cloned the human c-myc gene into the construct while at SJCRH. Dr. Cleveland obtained an MTA for this construct from SJCRH when he moved to the Scripps Research Institute, Florida.
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MSCV vector is described in; Hawley, R.G., Lieu, F.H.L., Fong, A.Z.C., and Hawley, T.S. Versatile retroviral vectors for potential use in gene therapy. Gene Ther. 1: 136-138, 1994.
Use of the construct is described in; Park, I., Zhao, R., West, J.A., Yabuuchi, A., Huo, H., Ince, T.A., Lerou, P.H., Lensch, M.W., and Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature, 451, 141-146, 2008.

Please note that the assembled sequence presented here is not 100% reflective of the actual sequence. See the Addgene diagnostic digest (found under the Resource Information section of the plasmid page) for additional information on the expected plasmid size. Addgene has found another HindIII site at the end of the MYC gene (see our quality control sequence).