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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5
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Backbone manufacturer(BD Pharmingen)
- Backbone size w/o insert (bp) 4754
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRaptor
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SpeciesH. sapiens (human)
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MutationS722A, S792A
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Entrez GeneRPTOR (a.k.a. KOG1, Mip1)
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Tag
/ Fusion Protein
- Myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is derived from Addgene #1859; two serine sites in this construct were mutated to alanine using Quickchange
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
myc-Raptor S722A/S792A was a gift from Reuben Shaw (Addgene plasmid # 18118 ; http://n2t.net/addgene:18118 ; RRID:Addgene_18118) -
For your References section:
AMPK phosphorylation of raptor mediates a metabolic checkpoint. Gwinn DM, Shackelford DB, Egan DF, Mihaylova MM, Mery A, Vasquez DS, Turk BE, Shaw RJ. Mol Cell. 2008 Apr 25. 30(2):214-26. 10.1016/j.molcel.2008.03.003 PubMed 18439900