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Addgene

pBABE myc-Raptor S722A/S792A
(Plasmid #18117)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18117 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-hygro
  • Backbone size w/o insert (bp) 5558
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Raptor
  • Species
    H. sapiens (human)
  • Mutation
    S722A, S792A
  • GenBank ID
    BC136654
  • Entrez Gene
    RPTOR (a.k.a. KOG1, Mip1)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene #1859 was mutated at two serine residues, then PCR amplified with primers containing EcoRI and XhoI, then ligated into pBABE-hygro and fully sequenced

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE myc-Raptor S722A/S792A was a gift from Reuben Shaw (Addgene plasmid # 18117 ; http://n2t.net/addgene:18117 ; RRID:Addgene_18117)

  • For your References section:

    AMPK phosphorylation of raptor mediates a metabolic checkpoint. Gwinn DM, Shackelford DB, Egan DF, Mihaylova MM, Mery A, Vasquez DS, Turk BE, Shaw RJ. Mol Cell. 2008 Apr 25. 30(2):214-26. 10.1016/j.molcel.2008.03.003 PubMed 18439900
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