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Addgene

pRQ-Id1HA
(Plasmid #18112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N/A
  • Backbone manufacturer
    Generated from multiple sources
  • Backbone size w/o insert (bp) 6633
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    STBL2 @ 30'C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Inhibitor of differentiation 1
  • Alt name
    id1
  • Alt name
    idb1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    443
  • GenBank ID
    NM_010495
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site gateway (unknown if destroyed)
  • 3′ cloning site Gateway (unknown if destroyed)
  • 5′ sequencing primer GCT CGT TTA GTG AAC CGT CA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRQ-Id1HA was a gift from Alex Swarbrick (Addgene plasmid # 18112 ; http://n2t.net/addgene:18112 ; RRID:Addgene_18112)

  • For your References section:

    Id1 cooperates with oncogenic Ras to induce metastatic mammary carcinoma by subversion of the cellular senescence response. Swarbrick A, Roy E, Allen T, Bishop JM. Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7. 10.1073/pnas.0801505105 PubMed 18378907
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