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Addgene

pcDNA3-Elafin
(Plasmid #18102)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18102 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Elafin
  • Alt name
    PI3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    368
  • Entrez Gene
    PI3 (a.k.a. ESI, SKALP, WAP3, WFDC14, cementoin)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Elafin was a gift from Ronny Drapkin (Addgene plasmid # 18102 ; http://n2t.net/addgene:18102 ; RRID:Addgene_18102)

  • For your References section:

    Elafin drives poor outcome in high-grade serous ovarian cancers and basal-like breast tumors. Labidi-Galy SI, Clauss A, Ng V, Duraisamy S, Elias KM, Piao HY, Bilal E, Davidowitz RA, Lu Y, Badalian-Very G, Gyorffy B, Kang UB, Ficarro S, Ganesan S, Mills GB, Marto JA, Drapkin R. Oncogene. 2015 Jan 15;34(3):373-83. doi: 10.1038/onc.2013.562. Epub 2014 Jan 27. 10.1038/onc.2013.562 PubMed 24469047
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