peT3a-AqAdk_MVGDH
(Plasmid
#18092)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18092 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET3A
- Backbone size w/o insert (bp) 5500
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameadenylate kinase
-
SpeciesA. aeolicus
-
Insert Size (bp)669
-
MutationTyr 52 changed to Cys, Val 145 changed to Cys, MetValGly added before initial Met of adenylate kinase sequence
-
GenBank IDO66490
-
Entrez Genekad
-
Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
peT3a-AqAdk_MVGDH was a gift from Dorothee Kern (Addgene plasmid # 18092 ; http://n2t.net/addgene:18092 ; RRID:Addgene_18092) -
For your References section:
Intrinsic motions along an enzymatic reaction trajectory. Henzler-Wildman KA, Thai V, Lei M, Ott M, Wolf-Watz M, Fenn T, Pozharski E, Wilson MA, Petsko GA, Karplus M, Hubner CG, Kern D. Nature. 2007 Dec 6. 450(7171):838-44. 10.1038/nature06410 PubMed 18026086