pcDNA3.2/v5-DEST hGlut9
(Plasmid
#18088)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 18088 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.2/V5-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlut9
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SpeciesH. sapiens (human)
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Entrez GeneSLC2A9 (a.k.a. GLUT9, GLUTX, UAQTL2, URATv1)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attB1 (not destroyed)
- 3′ cloning site attB2 (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.2/v5-DEST hGlut9 was a gift from Wolf Frommer (Addgene plasmid # 18088 ; http://n2t.net/addgene:18088 ; RRID:Addgene_18088) -
For your References section:
Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141