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Addgene

pcDNA3.2/v5-DEST hGlut4
(Plasmid #18087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glut4
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC2A4 (a.k.a. GLUT4)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2/v5-DEST hGlut4 was a gift from Wolf Frommer (Addgene plasmid # 18087 ; http://n2t.net/addgene:18087 ; RRID:Addgene_18087)

  • For your References section:

    Facilitative plasma membrane transporters function during ER transit. Takanaga H, Frommer WB. FASEB J. 2010 Aug . 24(8):2849-58. 10.1096/fj.09-146472 PubMed 20354141
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