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Purpose(Empty Backbone) This vector simultaneously expresses mCherry protein and shRNA in a Cre-dependent manner.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAAV-EF1a-DIO-EGFP
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Modifications to backboneGFP was replaced with mCherry, and the U6 promoter was added on the opposite side. In addition, HpaI and SpeI sequences capable of inserting shRNA were added after TATAlox.
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Vector typeMammalian Expression, AAV, RNAi, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer mCherry-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-R-Creon shRNA[Control] was a gift from Eun Mi Hwang (Addgene plasmid # 180775 ; http://n2t.net/addgene:180775 ; RRID:Addgene_180775) -
For your References section:
Improved AAV vector system for cell-type-specific RNA interference. Kim SC, Kim A, Park JY, Hwang EM. J Neurosci Methods. 2021 Dec 21:109452. doi: 10.1016/j.jneumeth.2021.109452. 10.1016/j.jneumeth.2021.109452 PubMed 34953938
Map uploaded by the depositor.
