pCAGGS-S-B.1.1.529-FLAG
(Plasmid
#180774)
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PurposeB.1.1.529 variant S-protein with c-terminal FLAG tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCAGGS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameS-protein B.1.1.529
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Alt namespike protein
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Alt nameomicron variant
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SpeciesSARS-CoV-2
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site Bglii (not destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer Bglob-PA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-S-B.1.1.529-FLAG was a gift from Daniel Conway (Addgene plasmid # 180774 ; http://n2t.net/addgene:180774 ; RRID:Addgene_180774)
