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Addgene

pcDNA3.1-α-COPI-WD40-Asp115Ala
(Plasmid #180760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    α-COPI-WD40-Asp115Ala
  • Species
    Schizosaccharomyces pombe
  • Mutation
    Encodes residues 1-327; mutations: Asp115Ala, Leu181Lys, Leu185Lys, Ile192Lys, Leu196Lys and Phe197Lys
  • GenBank ID
    NM_001021186
  • Promoter CMV
  • Tag / Fusion Protein
    • C-terminal strep tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer SV40 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-α-COPI-WD40-Asp115Ala was a gift from Syed Saif Hasan (Addgene plasmid # 180760 ; http://n2t.net/addgene:180760 ; RRID:Addgene_180760)

  • For your References section:

    An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking. Dey D, Singh S, Khan S, Martin M, Schnicker NJ, Gakhar L, Pierce BG, Hasan SS. Commun Biol. 2022 Feb 8;5(1):115. doi: 10.1038/s42003-022-03063-y. 10.1038/s42003-022-03063-y PubMed 35136165
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