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pGEX-2TK-WW-CR-W3083F
(Plasmid #18076)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18076 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2TK (Pharmacia)
  • Backbone manufacturer
    Phramacia
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dystrophin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    900
  • Mutation
    Second conserved Tryptophan (W) of the WW domain at amino acid position 3083 was changed to Phenylalanine (F)
  • GenBank ID
    NM_004006
  • Entrez Gene
    DMD (a.k.a. BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We obtained the partial cDNA for human dystrophin from ATCC. That cDNA served as a template for generation of constructs we produced in pGEX2TK

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2TK-WW-CR-W3083F was a gift from Marius Sudol (Addgene plasmid # 18076 ; http://n2t.net/addgene:18076 ; RRID:Addgene_18076)

  • For your References section:

    The WW domain of dystrophin requires EF-hands region to interact with beta-dystroglycan. Rentschler S, Linn H, Deininger K, Bedford MT, Espanel X, Sudol M. Biol Chem. 1999 Apr . 380(4):431-42. 10.1515/BC.1999.057 PubMed 10355629
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