pET30-T7-sfGFP
(Plasmid #180757)

• Purpose: pET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator
• Depositing Lab: Michael Black
• Publication: Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:895069. doi: 10.3389/fbioe.2022.895069. eCollection 2022.

Backbone

• Vector backbone: pET30a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5199
• Total vector size (bp): 5928
• Modifications to backbone: Removed lacO sequence, N-terminal His tag, and portion of the MCS to insert sfGFP with C-terminal S-tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: superfolder green fluorescent protein
• Alt name: sfGFP
• Insert Size (bp): 729
• Tag / Fusion Protein:
  • strep tag (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: derived from pJL1-sfGFP, a gift from Michael Jewett (Addgene plasmid # 102634)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry