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pET30-T7-lacO-His-sfGFP
(Plasmid #180754)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180754 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5315
  • Total vector size (bp) 6044
  • Modifications to backbone
    Removed portion of the MCS to insert sfGFP with C-terminal S-tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    superfolder green fluorescent protein
  • Alt name
    sfGFP
  • Insert Size (bp)
    729
  • Mutation
    G140C- Please see depositor comments
  • Tag / Fusion Protein
    • strep tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    derived from pJL1-sfGFP, a gift from Michael Jewett (Addgene plasmid # 102634)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms the G140C SNP in sfGFP does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-T7-lacO-His-sfGFP was a gift from Michael Black (Addgene plasmid # 180754 ; http://n2t.net/addgene:180754 ; RRID:Addgene_180754)

  • For your References section:

    Characterizing and Improving pET Vectors for Cell-free Expression. Jew K, Smith PEJ, So B, Kasman J, Oza JP, Black MW. Front Bioeng Biotechnol. 2022 Jun 23;10:895069. doi: 10.3389/fbioe.2022.895069. eCollection 2022. 10.3389/fbioe.2022.895069 PubMed 35814024
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