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Addgene

pMYT054
(Plasmid #180707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 180707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYTK095
  • Backbone manufacturer
    John Dueber
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMYT054 was a gift from Tom Ellis (Addgene plasmid # 180707 ; http://n2t.net/addgene:180707 ; RRID:Addgene_180707)

  • For your References section:

    A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. 10.1021/acssynbio.3c00423 PubMed 37930278
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