pMYT052
(Plasmid
#180705)
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Purpose(Empty Backbone) Assembly cassette with ConL7 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK095
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Backbone manufacturerJohn Dueber
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP expression in E. coli. Plasmid yields may drop after multiple passages or delayed miniprepping after the culture has saturated. We suggest streaking onto solid media to pick fresh colonies every time.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMYT052 was a gift from Tom Ellis (Addgene plasmid # 180705 ; http://n2t.net/addgene:180705 ; RRID:Addgene_180705) -
For your References section:
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. 10.1021/acssynbio.3c00423 PubMed 37930278