pMYT004
(Plasmid
#180657)
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PurposeEncodes pLacC as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 180657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK001
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Backbone manufacturerJohn Dueber
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepLacC
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMYT004 was a gift from Tom Ellis (Addgene plasmid # 180657 ; http://n2t.net/addgene:180657 ; RRID:Addgene_180657) -
For your References section:
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae. Shaw WM, Khalil AS, Ellis T. ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423. 10.1021/acssynbio.3c00423 PubMed 37930278