pLVX-mCherry-SPASTIN, F124D
(Plasmid #180648)

• Purpose: Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.
• Depositing Lab: Wesley Sundquist
• Publication: Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779.

Backbone

• Vector backbone: pLVX
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SPASTIN
• Species: H. sapiens (human)
• Mutation: Starts at M87, F124D mutation, has siRNA resistance to GAACAGUGUGAAAGAGCUA
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pLVX-Tight-mCherry-M87-SPASTIN-F124D.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Read the preprint on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.02.09.477148v1